Short read mapping pipeline pig: Difference between revisions

From HPCwiki
Jump to navigation Jump to search
No edit summary
Line 13: Line 13:


<source lang='bash'>
<source lang='bash'>
mysql -u ABGSAuser -h scomp1095.wurnet.nl -p ABGSAschema -e 'select ABG_individual_id from ABGSAschema_main where archive_name like "ABGSA03%" group by ABG_individual_id' >list300.txt
mysql -u ABGSAuser -h scomp1095.wurnet.nl -p ABGSAschema -e 'select ABG_individual_id from ABGSAschema_main where archive_name like "ABGSA0%" group by ABG_individual_id' >list.txt
cat list100.txt list200.txt list300.txt | sort | uniq >list100-200-300.txt
FILES=`cat list.txt`
FILES=`cat list100-200-300.txt`
for ID in $FILES; do python ABGC_mapping_v2.py -i $ID -a /lustre/nobackup/WUR/ABGC/shared/Pig/ABGSA/ -r /lustre/nobackup/WUR/ABGC/shared/Pig/Sscrofa_build10_2/Ensembl72/Sus_scrofa.Sscrofa10.2.72.dna.toplevel.fa -t 4; done
for ID in $FILES; do python ABGC_mapping_v2.py -i $ID -a /lustre/nobackup/WUR/ABGC/shared/Pig/ABGSA/ -r /lustre/nobackup/WUR/ABGC/shared/Pig/Sscrofa_build10_2/Ensembl72/Sus_scrofa.Sscrofa10.2.72.dna.toplevel.fa -t 4; done
</source>
</source>

Revision as of 19:52, 27 December 2013

The latest short-read mapping pipeline for the pig project is based on a Python3 script that creates a shell script that can subsequently be executed


Prerequisites

Basic execution

<source lang='bash'> (testenv)[megen002@nfs01 rundir]$ python ABGC_mapping_v2.py -i LW22F04 -a /lustre/nobackup/WUR/ABGC/shared/Pig/ABGSA/ -r /lustre/nobackup/WUR/ABGC/shared/Pig/Sscrofa_build10_2/Ensembl72/Sus_scrofa.Sscrofa10.2.72.dna.toplevel.fa -t 4 </source>

Automated runfile creation

<source lang='bash'> mysql -u ABGSAuser -h scomp1095.wurnet.nl -p ABGSAschema -e 'select ABG_individual_id from ABGSAschema_main where archive_name like "ABGSA0%" group by ABG_individual_id' >list.txt FILES=`cat list.txt` for ID in $FILES; do python ABGC_mapping_v2.py -i $ID -a /lustre/nobackup/WUR/ABGC/shared/Pig/ABGSA/ -r /lustre/nobackup/WUR/ABGC/shared/Pig/Sscrofa_build10_2/Ensembl72/Sus_scrofa.Sscrofa10.2.72.dna.toplevel.fa -t 4; done </source>

Output files

See also

External links