BLAST

Basic Local Alignment Search Tool, or BLAST, is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of different proteins or the nucleotides of DNA sequences. A BLAST search enables a researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble the query sequence above a certain threshold. Different types of BLASTs are available according to the query sequences. For example, following the discovery of a previously unknown gene in the mouse, a scientist will typically perform a BLAST search of the human genome to see if humans carry a similar gene; BLAST will identify sequences in the human genome that resemble the mouse gene based on similarity of sequence.

Module file

The module file can be found in this location:

 /cm/shared/apps/WUR/ABGC/modulefiles

<source lang='tcl'>

1. %Module1.0#######################################################################
   1. BLAST+ 2.2.28 modulefile

proc ModulesHelp { } {

       puts stderr "\tAdds blast+ v2.2.28 to your environment"

}

module-whatis "Adds blast+ v2.2.28 to your environment"

set blastplus_2228_root /cm/shared/apps/WUR/ABGC/blast/ncbi-blast-2.2.28+/bin/

prepend-path PATH $blastplus_2228_root

</source>

External links

• Maker homepage
• Maker tutorial