BLAST: Difference between revisions

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(Created page with "'''B'''asic '''L'''ocal '''A'''lignment '''S'''earch '''T'''ool, or '''BLAST''', is an algorithm for comparing primary biological sequence informatio...")
 
 
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'''B'''asic '''L'''ocal '''A'''lignment '''S'''earch '''T'''ool, or '''BLAST''', is an [[algorithm]] for comparing [[Primary structure|primary]] biological sequence information, such as the [[amino acid|amino-acid]] sequences of different [[protein]]s or the [[nucleotide]]s of [[DNA sequence]]s. A BLAST search enables a researcher to compare a query sequence with a library or [[database]] of sequences, and identify library sequences that resemble the query sequence above a certain threshold. Different types of BLASTs are available according to the query sequences. For example, following the discovery of a previously unknown gene in the [[Mus musculus|mouse]], a scientist will typically perform a BLAST search of the [[human genome]] to see if humans carry a similar gene; BLAST will identify sequences in the human genome that resemble the mouse gene based on similarity of sequence.
'''B'''asic '''L'''ocal '''A'''lignment '''S'''earch '''T'''ool, or '''[http://en.wikipedia.org/wiki/BLAST BLAST]''', is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of different proteins or the nucleotides of DNA sequences. A BLAST search enables a researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble the query sequence above a certain threshold. Different types of BLASTs are available according to the query sequences. For example, following the discovery of a previously unknown gene in the mouse, a scientist will typically perform a BLAST search of the human genome to see if humans carry a similar gene; BLAST will identify sequences in the human genome that resemble the mouse gene based on similarity of sequence.


== Module file ==
== Module file ==
The module file can be found in this location:
The module file can be found in this location:
   /cm/shared/apps/WUR/ABGC/modulefiles
   /cm/shared/modulefiles/SHARED/


<source lang='tcl'>
<source lang='tcl'>
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module-whatis  "Adds blast+ v2.2.28 to your environment"
module-whatis  "Adds blast+ v2.2.28 to your environment"


set            blastplus_2228_root        /cm/shared/apps/WUR/ABGC/blast/ncbi-blast-2.2.28+/bin/
set            blastplus_2228_root        /cm/shared/apps/SHARED/blast/ncbi-blast-2.2.28+/bin/


prepend-path    PATH            $blastplus_2228_root
prepend-path    PATH            $blastplus_2228_root


</source>
</source>
Please note that Maker requires the system tempdir to be in a non-NFS location. To be safe, best to put it in a local disc. For that reason the <code>TMPDIR</code> environment variable gets set to <code>/local</code>.  
 
== Installation details ==
Installed from pre-compiled binaries through link on the [http://www.repeatmasker.org/RMBlast.html RepeatMasker homepage].
 
== See also ==
== See also ==
* [[Globally_installed_software | Globally installed software]]
* [[ABGC_modules | ABGC specific modules]]


== External links ==
== External links ==
* [http://www.yandell-lab.org/software/maker.html Maker homepage]
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi Blast homepage]
* [http://gmod.org/wiki/MAKER_Tutorial_2013 Maker tutorial]
* [http://en.wikipedia.org/wiki/BLAST Blast on Wikipedia]
* [http://www.ncbi.nlm.nih.gov/books/NBK1763/#CmdLineAppsManual.Cookbook Blast Cookbook]

Latest revision as of 09:55, 15 March 2014

Basic Local Alignment Search Tool, or BLAST, is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of different proteins or the nucleotides of DNA sequences. A BLAST search enables a researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble the query sequence above a certain threshold. Different types of BLASTs are available according to the query sequences. For example, following the discovery of a previously unknown gene in the mouse, a scientist will typically perform a BLAST search of the human genome to see if humans carry a similar gene; BLAST will identify sequences in the human genome that resemble the mouse gene based on similarity of sequence.

Module file

The module file can be found in this location:

 /cm/shared/modulefiles/SHARED/

<source lang='tcl'>

  1. %Module1.0#######################################################################
    1. BLAST+ 2.2.28 modulefile

proc ModulesHelp { } {

       puts stderr "\tAdds blast+ v2.2.28 to your environment"

}

module-whatis "Adds blast+ v2.2.28 to your environment"

set blastplus_2228_root /cm/shared/apps/SHARED/blast/ncbi-blast-2.2.28+/bin/

prepend-path PATH $blastplus_2228_root

</source>

Installation details

Installed from pre-compiled binaries through link on the RepeatMasker homepage.

See also

External links